FIGURE 1.

Inhibition of NAMPT by FK866 led to NAD⁺ and ATP depletion and subsequent cell death. A2780 cells were grown and treated with FK866 (100 nm) in duplicate as described (“Experimental Procedures”). After the treatment, cells were processed for the analysis of NAD⁺ by LC-MS and the fluorescence assays, ATP by ATPlite and cell death by CellTiter-Fluor™ as described (“Experimental Procedures”). Error bars represent S.D.