FIGURE 4.

A, Pmat cDNA amplified from total RNA of WT and Pmat^−/− mouse brain. Lane 1, molecular mass marker; lane 2, WT mouse; lane 3, Pmat^−/− mouse. The expected cDNA sizes are 1627 bp for the WT allele and 932 bp for Pmat null allele. B, functional analysis of cells stably transfected with cDNA encoding the Slc29a4 gene product either from WT or KO mice. cDNAs amplified in A were cloned into the pcDNA5/FRT expression vector and stably expressed in Flp-in HEK293 cells. Uptake studies were conducted with cells stably transfected with product amplified from WT or KO brain along with the pcDNA5 empty vector. The PMAT substrates MPP⁺, dopamine, and 5-HT were used. The results represent the means ± S.D. of three independent experiments. *, p < 0.05 compared with pcDNA5 vector control.