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. 2012 Dec 19;288(5):3545–3552. doi: 10.1074/jbc.M112.442350

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FIGURE 1. — Purified Syn5 RNAP and transcription on Syn5 DNA. A, SDS-PAGE gel of purified non-tagged Syn5 RNAP (lane 1) and N-terminal His-tagged Syn5 RNAP obtained after a Ni-NTA-agarose column (lane 2), gel filtration column (lane 3), and cellulose phosphate column (lane 4). Purified T7 RNAP is shown for comparison. Proteins were stained with Coomassie Blue. B, 10% denaturing TBE-urea gel of radioactive labeled transcription products generated by Syn5 RNAPs on Syn5 genomic DNA. The lanes in B correspond to transcription by the proteins purified in A.