FIGURE 1.

Caspase 8 is up-regulated in Smad7-expressing MCF7 cells. A, total RNA was isolated from control or FLAG-Smad7-expressing MCF7 cells, and semi-quantitative RT-PCR was performed using caspase 8 (Casp8)- or Smad7-specific primers. GAPDH was used as internal control. B, to confirm the microarray and RT-PCR results, protein lysates were prepared from MCF7 cells, and the expression of caspase 8 or FLAG-Smad7 was detected using specific antibodies. The loading amount was normalized with β-actin antibody.