FIGURE 4.

Knockdown of PTEN, AMPK, and PKR gene expression reduced ARV σC protein synthesis and viral yield. DF-1 and Vero cells transfected with respective shRNA or scramble shRNA were mocked or infected with ARV at m.o.i. of 10 for 24 h. Cell lysates were analyzed by Western blotting with antibodies against PTEN (A), AMPK (B), and PKR (C), as well as ARV σC, LC3-II or β-actin, as indicated. D, supernatants of ARV-infected and shRNA-transfected cells in each well were harvested at 24 hpi for viral titration. Each value represents the mean of three independent experiments ± S.D.