Blockade of p53 by p53 shRNA-inhibited p17 up-regulated PTEN, Beclin 1, and LC3-II. Vero and DF-1 cells were transfected with p53 shRNA, pcDNA3.1, and p17 for 24 h followed by Western blot analysis. The Western blot results of different combinations of treatments in Vero and DF-1 cells are shown. Phosphorylation and protein levels were determined by immunoblotting with the appropriate antibodies, as indicated. Similar results were obtained in three independent experiments.