FIGURE 7.

Knockdown of PTEN, AMPK, PKR, and ATG7 reduced the level of LC3-II protein in p17-transfected cells. DF-1 and Vero cells were transfected with the respective shRNA, scrambled shRNAs, and mock transfection, respectively, for 24 h. Cell lysates were analyzed by Western blotting with antibodies against PTEN (A), AMPK (B), PKR (C), PKR and AMPK (D), PKR and Beclin 1 (E), ATG7 (F), and β-actin, as indicated. G, PTEN, AMPK, and PKR signalings were suppressed in p17 shRNA-treated cells. Similar results were obtained in three independent experiments.