FIGURE 1.

Localization of Cx32WT and selected mutants at the Arg⁷⁵ position expressed in HeLa cells. HeLa cells were transiently transfected with DNA coding for the noted variants of Cx32 and stained with mouse anti-connexin antibodies in conjunction with an Alexa Fluor 594 secondary. As shown, Cx32WT and the R75L mutant show elongated puncta at intercellular appositions (arrows), consistent with gap junction plaques. R75P, R75Q, and R75L all exhibit a pattern of staining consistent with localization to the Golgi compartment. Note that the expression level for the R75W mutant is very low. Scale bar, 20 μm.