TABLE 1.

Recombinant proteins used in this study

Plasmida	Protein	Host vector
pSur1-Ce	FLAG-Sur1-Cerulean	pECE
pCi-Trpm4	Citrine-Myc-Trpm4	pCMV-Tag3C
pTrpm4-Ci	Myc-Trpm4-Citrine	pCMV-Tag3C
pCi-Kir6.2	Citrine-Myc-Kir6.2	pCMV-Tag3C
pCi-Kir2.1	Citrine-Myc-Kir2.1	pCMV-Tag3C
pcDNA-His6-Sur1b	His6-Sur1	pcDNA3.1
pECE-FLAG-Sur1c	FLAG-Sur1	pECE
pMyc-Trpm4	Myc-Trpm4	pCMV-Tag3C
pMyc-Kir6.2	Myc-Kir6.2	pCMV-Tag3C
pMyc-Hif1α	Myc-Hif1α	pCMV-Tag3C
pTrpm4-EGFPd	Trpm4-EGFP	pEGFP-N1
pIRES-Sur1-Trpm4	His6-Sur1-Trpm4	pEF1α-IRES-AcGFP1
pHygroB-Sur1	His6-Sur1	pcDNA-His6-Sur1

^a Fusion to the N terminus of Sur1 or Trpm4 is indicated by listing the adduct first; fusion to the C terminus of Sur1 or Trpm4 is indicated by listing the adduct second.

^b Gift of Dr. Joseph Bryan, Pacific Northwest Diabetes Research Institute, Seattle, WA (11, 12).

^c Gift of Dr. Show-Ling Shyng, Oregon Health and Science University, Portland, OR.

^d See Gerzanich et al. (28).