Figure 2. Expression of HLA-DR on basophils in the circulation and in the cells isolated by two different kits.

(a) Red blood cells in the buffy bags were lysed and stained for HLA-DR and CD123 (b) Basophils were isolated either by EasySep Human Basophil enrichment kit that does not deplete HLA-DR expressing basophils or by basophil isolation kit II. The expression of HLA-DR and CD123 was analyzed by flow cytometry.