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. 2013 Feb 1;3:1197. doi: 10.1038/srep01197

Figure 5. ADSCs decrease both the extracellular and intracellular Aβ levels of N2a cells.

Figure 5

(A) Schematic representation of the co-culture experiments of N2a cells with ADSCs or BM-MSCs using cell culture inserts. Secreted Aβ40 (B) and Aβ42 (C) levels were measured using the collected CM in the bottom chambers by ELISA. Each value was normalized to the protein content of N2a cell lysates. Data are the mean ± S.D. (n = 4). (D) Intracellular Aβ42 levels were measured using the N2a cell lysates by ELISA. Each value was normalized to the protein content of N2a cell lysates. (E) Intracellular Aβ42 levels were compared in co-cultures of ADSCs and N2a cells in the presence and absence of thiorphan. Each value is shown as the relative level to the control. Data are the mean ± S.D. (n = 4).