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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Cereb Cortex. 2011 Aug 19;22(6):1333–1342. doi: 10.1093/cercor/bhr224

Figure 1.

Figure 1

Hyperconnectivity of layer 5 pyramidal neurons in mPFC of 2–3 week but not 3- to 5-week-old Fmr1-KO mice. (A) Hexa-patch recorded cluster of 6 biocytin-stained pyramidal neurons (scale bar 25 μm). Inset top, 8 AP stimulation at 30 Hz with single test pulse 500 ms later, and inset bottom, corresponding EPSPs from monosynaptic connection. (B) Connectivity outline for examples of a recorded WT (2 of 30 possible synaptic connections) and an Fmr1-KO cluster (6 of 30 possible synaptic connections), calculated from a connectivity matrix of all possible synaptic connections for one example cluster (see Supplementary Fig. 1). (C) CP distributions for the clusters of Fmr1-KO (N = 432 neurons) and WT neurons (N = 466), up to 125 μm apart, were significantly different (Wilcoxon sum rank test, **P < 0.01). (D) CP distributions for the clusters of Fmr1-KO (N = 384 neurons) and WT neurons (N = 296), up to 125 μm apart, were not significantly different from each other (Wilcoxon sum rank test, P = 0.5). Individual values listed for both age groups in Supplementary Table 1.