Figure 5. Pretreatment with BITC or SFN sensitizes HeLa cells to apoptosis induced by CDDP.
A, cells were treated with 15 μM BITC or 40 μM SFN for the indicated times. Cleavage of caspase substrate PARP was assessed by Western Blot analysis using an anti-PARP antibody. B, effect of the combined exposure to BITC (5 μM) and CDDP (10μM) on the cleavage of caspase substrate PARP. C, effect of the combined exposure to SFN (20 μM) and CDDP (10 μM) on the cleavage of caspase substrate PARP.