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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Curr Protoc Microbiol. 2011 Feb;CHAPTER:Unit–12C.4. doi: 10.1002/9780471729259.mc12c04s20

Table 1.

Commonly used B. burgdorferi senus stricto strains.

Strain Description and Reference


B31 Infectious low-passage wildtype strain isolated from Ixodes dammini and cloned by limiting dilution (Barbour, 1984; Burgdorfer et al., 1982).
297 Infectious low-passage wildtype strain isolated from human spinal fluid (Steere et al., 1983).
cN40 Infectious clonal low-passage wildtype strain isolated from adult I. dammini (Barthold et al., 1990, 1988)
CA-2-87 I. pacificus isolate from Northern California (Schwan et al., 1988)
JD-1 Isolated and maintained in Ixodes ticks (Piesman et al., 1987)
B31 derivatives
B31 MI Derived from tick isolated B31 and passed through mouse (Casjens et al., 1997).
B31-A3 Clonal B31 infectious and low-passage; plasmids and infectivity maintained with transformation of shuttle or suicide vectors (Elias et al., 2002)
MSK5 Clonal infectious low-passage strain isolated from C3H/HeN mouse skin (Labandeira-Rey and Skare, 2001).
MSK7 Clonal infectious low-passage strain isolated from C3H/HeN mouse skin, lacking lp28-4 (Labandeira-Rey and Skare, 2001).
MSK10 Clonal low-passage strain unable to establish persistant infection; lacking lp28-1 isolated from C3H/HeN mouse skin (Labandeira-Rey and Skare, 2001).
ML23 Clonal noninfectious low-passage strain lacking lp25 isolated from nonclonal P48 B31 culture (Labandeira-Rey and Skare, 2001).
5A# Clonal isolates with variable plasmid composition and infectivity (Purser and Norris, 2000).
5A4 Clonal infectious isolate containing all plasmids (Purser and Norris, 2000).
5A10 & 5A14 Clonal infectious isolate lacking lp25 and lp56 (Purser and Norris, 2000).
5A18 Clonal infectious isolate lacking lp28-4 and lp56 (Purser and Norris, 2000).
5A4 NP1 Clonal infectious isolate containing all plasmids(5A4) and bbe02 distrupted with PflgB-kanR (Kawabata et al., 2004)
5A18 NP1 5A18 with bbe02 distrupted by a PflgB-kanR cassette (Kawabata et al., 2004)
297 derivatives
BbAH130 Clonal infectious low-passage isolate from plating polyclonal 297 (Yang et al., 2004).
PL133 Clonal infectious lowpassage strain isolated from ear punch biopsies of mice infected with polyclonal 297 (Revel et al., 2005).
BbDTR630 Derived from PL133 with a PflgB-kanR cassette cloned into lp25 (Blevins et al., 2007).
297-LK Non-infectious clonal isolate derived from AH130 and is lacking lp28-1; lacI and PflgB-kan cloned into bbe02 on lp25 (Gilbert et al., 2007)
c155 Infectious low-passage isolated from nonclonal 297 by two rounds of plating and colony extraction (Eggers et al., 2002).
CE162 Clonal infectious low-passage derived from wildtype 297 (Caimano et al., 2004).
cN40 derivatives
D10/E9 Infectious low-passage clonal isolate (Parveen et al., 2006).