Table 1.
Commonly used B. burgdorferi senus stricto strains.
Strain | Description and Reference |
---|---|
B31 | Infectious low-passage wildtype strain isolated from Ixodes dammini and cloned by limiting dilution (Barbour, 1984; Burgdorfer et al., 1982). |
297 | Infectious low-passage wildtype strain isolated from human spinal fluid (Steere et al., 1983). |
cN40 | Infectious clonal low-passage wildtype strain isolated from adult I. dammini (Barthold et al., 1990, 1988) |
CA-2-87 | I. pacificus isolate from Northern California (Schwan et al., 1988) |
JD-1 | Isolated and maintained in Ixodes ticks (Piesman et al., 1987) |
B31 derivatives | |
B31 MI | Derived from tick isolated B31 and passed through mouse (Casjens et al., 1997). |
B31-A3 | Clonal B31 infectious and low-passage; plasmids and infectivity maintained with transformation of shuttle or suicide vectors (Elias et al., 2002) |
MSK5 | Clonal infectious low-passage strain isolated from C3H/HeN mouse skin (Labandeira-Rey and Skare, 2001). |
MSK7 | Clonal infectious low-passage strain isolated from C3H/HeN mouse skin, lacking lp28-4 (Labandeira-Rey and Skare, 2001). |
MSK10 | Clonal low-passage strain unable to establish persistant infection; lacking lp28-1 isolated from C3H/HeN mouse skin (Labandeira-Rey and Skare, 2001). |
ML23 | Clonal noninfectious low-passage strain lacking lp25 isolated from nonclonal P48 B31 culture (Labandeira-Rey and Skare, 2001). |
5A# | Clonal isolates with variable plasmid composition and infectivity (Purser and Norris, 2000). |
5A4 | Clonal infectious isolate containing all plasmids (Purser and Norris, 2000). |
5A10 & 5A14 | Clonal infectious isolate lacking lp25 and lp56 (Purser and Norris, 2000). |
5A18 | Clonal infectious isolate lacking lp28-4 and lp56 (Purser and Norris, 2000). |
5A4 NP1 | Clonal infectious isolate containing all plasmids(5A4) and bbe02 distrupted with PflgB-kanR (Kawabata et al., 2004) |
5A18 NP1 | 5A18 with bbe02 distrupted by a PflgB-kanR cassette (Kawabata et al., 2004) |
297 derivatives | |
BbAH130 | Clonal infectious low-passage isolate from plating polyclonal 297 (Yang et al., 2004). |
PL133 | Clonal infectious lowpassage strain isolated from ear punch biopsies of mice infected with polyclonal 297 (Revel et al., 2005). |
BbDTR630 | Derived from PL133 with a PflgB-kanR cassette cloned into lp25 (Blevins et al., 2007). |
297-LK | Non-infectious clonal isolate derived from AH130 and is lacking lp28-1; lacI and PflgB-kan cloned into bbe02 on lp25 (Gilbert et al., 2007) |
c155 | Infectious low-passage isolated from nonclonal 297 by two rounds of plating and colony extraction (Eggers et al., 2002). |
CE162 | Clonal infectious low-passage derived from wildtype 297 (Caimano et al., 2004). |
cN40 derivatives | |
D10/E9 | Infectious low-passage clonal isolate (Parveen et al., 2006). |