Table 2.

Plasmids for genetic manipulation of B. burgdorferi

Plasmid	Selectiona	Description and Source
Shuttle vectors
pBSV2	kanR	Shuttle vector containing three ORF’s from cp9 required for plasmid replication and distribution (Stewart et al., 2001).
pBBE22	kanR	pBSV2 containing bbe22/pncA fragment to restore infectivity to strains lacking lp25 (Purser et al., 2003).
pBBE22gate	kanR	Modified pBBE22 containing Gateway destination vector attR1 and attR2 sites from Invitrogen’s pDEST17 (Weening et al., 2008).
pKFSS1	strepR	Streptomycin resistant borrelial shuttle vector derived from pBSV2 (Frank et al., 2003).
pKFSS1gate	strepR	Modified pKFSS1 containing Gateway destination vector attR sites coned into the SphI site within the MCS (unpublished)
pCADDY	strepR	Shuttle vector derived from pBBE22; replacing the kanamycin resistance with streptomycin resistant gene (Hyde et al., 2009).
pCADDYgate	strepR	Derived from pBBE22gate (Weening et al., 2008); replacing the kanamycin resistance with streptomycin resistant determinant (unpublished)
pJD1	strepR	pKFSS1 derivative with reduced PflgB-aadA region (Blevins et al., 2007).
pJD7	strepR	pJD1 derivative with terminator inserted and Plac removed (Blevins et al., 2007).
pJD44	kanR	pJD7 derived plasmid replaced specR/strepR with kanR (Blevins et al., 2007)
pBSV2G	gentR	Gentamicin resistant shuttle vector derived from pBSV2 (Elias et al., 2003).
pBSV2Ggate	gentR	Modified pBSV2G containing Gateway destination vector attR sites at SphI site in MCS (unpublished)
pBSV2SynG	courR	Shuttle vector derived from pBSV2 encoding a synthetic gyrB (Elias et al., 2003)
pCE310	kanR	Shuttle vector with cp32 loci required for replication and distribution (Eggers et al., 2002).
pCB52	kanR	Contains replicon and telomere ends from lp17; replicates as circular plasmids in E. coli and as a linear plasmid in B. burgdorferi (Beaurepaire and Chaconas, 2005).
pBSV17	kanR	Shuttle vector derived from pBSV2 with the gene required for replication from lp17 (Beaurepaire and Chaconas, 2005).
pGK12	ermR	Broad-host plasmid able to propagate in Gram-positive and Gram-negative bacteria including B. burgdorferi (Sartakova et al., 2003).
Allelic exchange vectors
pJH333	gentR	A multicloning site and PflgB-gentR cassette flanked by DNA regions for allelic exchange into the borrelial chromosome between bb0445 and bb0446; based on the construct reported by Li et al., 2007 (unpublished)
pMC1667	noneb	Suicide vector with multicloning site flanked by cp26 regions forallelic exchange (Dunham-Ems et al., 2009).
pMC1916	gentR	Suicide vector derived from pMC1667 with PflgB-gentR and PflaB-gfp in divergent orientation are flanked by cp26 regions for allelic exchange (Dunham-Ems et al., 2009).
pSPC-G	gentR	Suicide vector with PflaB-gentR and PflaB-gfp in divergent orientation are flanked by cp26 regions for allelic exchange (Kenedy et al., 2009).

^aSelection is in reference to antibiotic resistance in Borrelia burgdorferi only; antibiotic resistance for usage in E. coli is not indicated

^bNo selectable marker is incorporated for usage in B. burgdorferi; plasmid does confer resistance to kanamycin and ampicillin for selection in E. coli