Figure 3. H2009.1 tetrameric peptide liposomes display specificity for α_vβ₆-expressing H2009 cells compared to αvβ6-low-expressing H1299 cells.

Cells were incubated with different liposome formulations for 1h and liposome accumulation in cells measured using flow cytometry. A total of 10,000 cells were counted per treatment group and doxorubicin fluorescence was measured (excitation 488nm, emission 550-600nm). Representative flow cytometry analyses for H2009 cells are shown in panels a-d and for H1299 cells are shown in panels e-h. Panels d & h show the averages from 3 independent flow assays in which the mean fluorescence intensities of the H2009.1 tetrameric and scH2009.1 tetrameric liposomes were normalized to the mean fluorescence intensity of the naked liposomes.