Figure 4. Time course for release of doxorubicin from 1.3% H2009.1 Tetrameric Liposomes.
Cells were treated with either free doxorubicin or 1.3% H2009.1 tetrameric liposomal doxorubicin for 1 hour before the drug was removed. Doxorubicin fluorescence was observed on a Nikon TE2000-E microscope at 60X magnification at the indicated time after initial treatment. Bright field and fluorescence images are overlaid to show the overall cell morphology.