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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Mol Endocrinol. 2012 Dec 31;50(1):10.1530/JME-12-0201. doi: 10.1530/JME-12-0201

Fig. 3. Detection of Δ7/11 and prolactin interactions.

Fig. 3

The direct interaction of Δ7/11 and recombinant human prolactin measured by solution-phase proximity ligation assays; SF1a and prolactin antibodies were linked to DNA-strands, upon proximal binding of the antibodies to their respective target proteins, the DNA strands ligate and the PCR amplicon is detected using quantitative real-time PCR. (A) Representative data of Δ7/11 and prolactin interactions using a Δ7/11 specific probe. (B) Representative data of Δ7/11 and prolactin interactions using a hPrl-specific probe. Data represent average ΔCt of a minimum of three replicates +/− SD.