Table 1.
Perturbation Type | Construct/Expression Assayed | # Expts |
Peak Output Sampled (hpf) |
Relative Expression (Avg) |
---|---|---|---|---|
Gcm site deleted a | 3 | 36,36,44 | 49% | |
cis-perturbations | Six1 site mutated a | 4 | 36,37,40,44 | 16% |
Gcm::gfp BAC, Six1 site mutated b | 1 | 28 | 40% | |
Intact late module + Six1 MO c | 3 | 40,40,40 | 30% | |
trans-perturbations | Gcm site del late module + Six1 MO c | 2 | 40,35 | 20% |
Gcm mRNA + Six1 MO c | 4 | 35,40,28,30 | 36% | |
Intact late module + gataE MO c | 2 | 32,32 | 20% |
Expression relative to:
intact late module;
intact gcm::gfp BAC;
same as shown, without MO inject