Fig. 6.

Ectopic repression mediated by SEs. (A-D) Brinker expression was monitored by lacZ reporters (green) and is shown with DAPI staining (light blue). The B14 reporter was Smad2 dependent (A,B) and the E14 brinker reporter lacking the SE was not Smad2 dependent (C,D). (E-H) A pentagone reporter with intact SEs lost expression in a Smad2 mutant (E,F) but a reporter with mutation of the SEs (pentΔSE) was no longer Smad2 dependent (G,H). Relationship between SE and ectopic silencing in the absence of Smad2 is summarized for each reporter. Wing discs are oriented with the distal end up and the anterior to the left. The green channel was captured and is displayed with matching settings for panels A and B and for E and F. Scale bars: 50 μm.