Figure 6. Platelet ITAM signaling is critical for the maintenance of vascular integrity during inflammation.

(A and B) Hemorrhage in skin lesions of TP hIL-4Rα/GPIbα–Tg mice reconstituted or not with the indicated platelet preparations (8 × 10⁸ platelets/mouse), with or without treatment with JAQ1 antibody (n = 15–20 spots per group). (A) Hb levels 4 hours after rpA challenge. Intradermal injection of PBS (without rpA) is also shown. (B) Representative images of lesions (dashed outlines and arrowheads). (C and D) LPS-induced inflammation in lungs of TP hIL-4Rα/GPIbα–Tg mice reconstituted or not with the indicated platelet preparations (8 × 10⁸ platelets/mouse), with or without treatment with JAQ1 antibody (n = 3–5 per group). (C) Hb levels in BAL after LPS challenge. Intradermal injection of PBS (without LPS) is also shown. (D) Representative images of BAL. **P < 0.01, ***P < 0.001 versus WT platelets; ^#P < 0.001 versus no platelet reconstitution.