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. 2013 Jan 2;123(2):782–786. doi: 10.1172/JCI65863

Figure 2. OPC cultures and in vitro BBB model.

Figure 2

(A) Gelatin zymography showed that MMP9 but not MMP2 was secreted from cultured rat OPCs by treatment with IL-1β (1-hour treatment at 100 ng/ml followed by 23-hour incubation under normal culture conditions). (B) We prepared conditioned medium from normal OPCs (OPC-CM) or IL-1β–treated OPCs (OPC′-CM), and then added them to cerebral endothelial RBE.4 cells. (C–E) Twenty-four hours after treatment with OPC′-CM degraded the tight-junction protein ZO-1 in RBE.4 cells. n = 3. *P < 0.05. (F and G) Compared with OPC-CM, OPC′-CM increased both endothelial permeability and neutrophil filtration. Importantly, BBB breakdown was reduced by cotreatment with MMP inhibitor GM6001 (10 μM). Ve, vehicle; GM, GM6001. n = 3. *P < 0.05.