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. 2013 Jan 2;123(2):712–726. doi: 10.1172/JCI65728

Figure 2. Ectopic lymphoid structures are associated with immune control during TB in the NHP model of Mtb infection.

Figure 2

NHPs aerosol infected with Mtb CDC1551 exhibited either L-TB or A-TB as described in Methods. Thirteen weeks after infection or at necropsy, lung FFPE serial sections were stained with H&E (A) or analyzed by immunofluorescence for PCNA, CD20 (A and B), or CD3, CXCR5, IgD; CD3, CD21 (B). All sections were counterstained with DAPI (blue). GC, containing large, proliferating B blasts (PCNA+CD20+), is outlined in dashed yellow lines. Yellow arrows point to typical CD3+CXCR5+ T cells within lymphoid structures. The images shown are from a typical representative section. Number of granulomas (C), total area occupied by granulomas (D), percentage of granuloma area occupied per biopsy (E), and average size and percentage of area occupied by B cell lymphoid follicles (F and G) were determined with the morphometric tool of the Zeiss Axioplan microscope. Original magnification, ×200, unless otherwise indicated. The data points represent the mean (±SD) of values from 6–15 NHPs (A–G). **P = 0.005, ***P = 0.0005.