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. 2013 Jan 9;123(2):812–822. doi: 10.1172/JCI64093

Figure 7. Influence of aldosterone on TNFA expression and osteoinductive signaling in HAoSMCs.

Figure 7

Arithmetic mean ± SEM (n = 6; arbitrary units) of mRNA levels encoding (A) TNFA, (B) MSX2, (C) CBFA1, and (D) ALPL in HAoSMCs after 24-hour treatments with vehicle alone (Control, white bars), with 100 nM aldosterone alone (Aldo, black bars), with 100 nM aldosterone and 10 μM spironolactone (Aldo+Spiro, dark gray bars), or with 10 μM spironolactone alone (Spiro, light gray bars). (E) Arithmetic mean ± SEM (n = 4; U/mg protein) of ALP activity of whole cell extracts from HAoSMCs after 7-day treatments with vehicle alone (white bar), with 100 nM aldosterone alone (black bar), with 100 nM aldosterone and 10 μM spironolactone (dark gray bar), or with 10 μM spironolactone alone (light gray bar). #P < 0.05, ##P < 0.01, compared with HAoSMCs treated with 100 nM aldosterone alone; *P < 0.05, **P < 0.01, compared with HAoSMCs treated with vehicle alone.