Figure 9. Cd28-ΔTregs have defective accumulation and function in vivo.

(A and B) Cd28^fl/flFoxp3^Cre female mice were transplanted with BALB/c body skin. WT and Cd28-ΔTregs from the blood were analyzed before or 8 days after transplantation. The percentage of increase of Tregs was analyzed in B, and each dot represents 1 mouse (horizontal bars indicate the mean). (C) Clinical scores of rMOG/CFA plus pertussis toxin–induced EAE in female WT Cd28^+/+ × FOXP3^{YFP-Cre/YFP-Cre} (WT) or Cd28^fl/fl × FOXP3^{YFP-Cre/YFP-Cre} (Cd28-ΔTreg) mice. A total of 7 mice in each group are shown. Data represent mean ± SEM.