Figure 6. RAD001 treatment decreases tumor cell proliferation and induces tissue hypoxia.

(A, C, E, and F) Representative immunostaining for (A) BrdU, (C) active caspase-3, (E) CD31, and (F) hypoxyprobe-1 (HP1) on sections of unaffected antra and tumors from 19-week-old gp130^FF mice collected after 6 weeks of RAD001 (10 mg/kg) treatment. (C) Apoptotic cells are indicated by arrows. Scale bar: 10 μm (insets in C); 100 μm (A, C, E, and insets in F); 500 μm (F). Also refer to Supplemental Figure 8. (B and D) Immunoblot analysis of unaffected antra and pooled tumors from individual 19-week-old gp130^FF mice collected after 6 weeks of RAD001 (10 mg/kg) treatment.