Fig. 1.

Representative carbenoxolone-sensitive WC currents recorded from cells of different lines transfected with the pIRES2-EGFP-Panx1 plasmid, including neuroblastoma SK-N-SH (A), CHO (B), and HEK-293 (C) cells. (A–C) Cells were held at −50 mV and polarized by 100-ms (SK-N-SH) or 50-ms (CHO and HEK-293) voltage pulses between −70 and 80 mV [upper panel in C]. Bottom panels: whole-cell (WC) currents were recorded in the presence of 25 µM carbenoxolone (CBX). In all cases, cells were perfused with the solution (mM): 140 NaCl, 2 KCl, 1 CaCl₂, 1 MgCl₂, 10 HEPES-NaOH; the patch pipette contains (mM): 100 CsCl, 0.5 MgCl₂, 10 BAPTA-40 CsOH, 10 mM HEPES-CsOH. (D–F) I–V curves of steady-state currents shown in A–C, respectively. The current values for generating I–V curves were measured at the moments indicated by the symbols above the current traces. (G–I) I–V curves of Panx1 currents with different anions in the bath, as indicated in G. In all cases, 140 mM NaCl+2 mM KCl in the bath was substituted for 140 mM Na-gluconate+2 mM K-gluconate or 140 mM NaMeSO₃+2 mM KMeSO₃.