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. 2013 Feb;23(2):260–269. doi: 10.1101/gr.138032.112

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 2. — Changes in H3K27me3 associated with changes in gene expression in HGPS. (A) Scatterplot of Log2 ratio expression changes between HGPS and Age Control (y-axis) and HGPS and Father (x-axis). Red and green boxes indicate genes considered “up-regulated” and “down-regulated” (more than fourfold), respectively, for subsequent analyses. (B) H3K27me3 changes between Father and HGPS in the 25-kb bin overlapping each gene are plotted on the y-axis for genes that are up-regulated (left) or down-regulated (right). H3K27me3 tends to decrease in HGPS for up-regulated genes and increase for down-regulated genes. (C) Examples of H3K27me3 changes for genes that are up-regulated (left, PDE3A and PTGS2) and that are down-regulated (right, SALL1) according to the expression microarray data in normal vs. HGPS fibroblasts. (D) Quantitative RT-PCR results confirm expression microarray results for these three example genes (*) P < 0.05; (**) P < 0.01 in unpaired, one-tailed Student's t-tests.