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. 2013 Feb;23(2):292–299. doi: 10.1101/gr.137224.112

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 1. — Identification of small RNAs generated from the EGFP-neo knock-in sequence. A small RNA library prepared from 5-wk-old EGFP-neo/+ testes was subjected to high-throughput sequencing. (A) Distribution of endogenous small RNA sequences across the Watson and Crick clusters (mm9 chr.17: 27,409,752-27,511,260) in wild-type control (+/+) and EGFP-neo/+ testes. The data is shown for 100-bp windows across the region (top). The precise read numbers are also shown (bottom). The overall patterns in the wild-type and EGFP-neo^+/– testes were very similar. (B) Distribution of small RNA sequences mapped within EGFP-neo. The small RNA read number for each 100-bp window is shown across the EGFP-neo fragment (top). The total read number for each strand of each gene is also shown (bottom).