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. 2012 Nov 29;22(5):890–903. doi: 10.1093/hmg/dds495

Figure 2.

Figure 2.

PrP-floxed-SCA7-92Q BAC mice exhibit PC degeneration, but normal BG at early symptomatic stage. Calbindin immunostaining of sagittal cerebellar sections from 20-week-old mice reveals more extensive PC dendritic branching and a thicker molecular layer in (A) non-transgenic mice in comparison to (B) PrP-floxed-SCA7-92Q BAC mice. Scale bar, 100 µm. (C) Quantification of the cerebellar molecular layer thickness reveals thinning of the molecular layer in PrP-floxed-SCA7-92Q BAC mice (n = 6) compared with non-transgenic mice (n = 4; two-tailed t-test, unpaired; P < 0.01). GFAP immunostaining of sagittal cerebellar sections indicates a similar cellular appearance and staining intensity for (D) non-transgenic mice and (E) PrP-floxed-SCA7-92Q BAC mice at 20 weeks of age. Scale bar, 25 µm. Quantification of BG process numbers demonstrates that the number of GFAP-positive processes is comparable for PrP-floxed-SCA7-92Q BAC mice (n = 6) and non-transgenic mice (n = 4–5) at (F) 20 weeks of age or (G) 25 weeks of age (two-tailed t-test, unpaired). Error bars = SEM.