Figure 3.

Cre-mediated excision of mutant ataxin-7 in PrP-floxed-SCA7-92Q BAC mice. (A) Outline of the breeding and treatment paradigm employed in this study. CAGGS-Cre-ER™ and non-transgenic animals were pooled together in one group designated as ‘SCA7-negative’. (B) Quantitative real-time RT–PCR analysis reveals that ataxin-7-92Q transgene expression is reduced by ∼50% in bigenic mice induced to express Cre recombinase in the nucleus (n = 3/group; P < 0.01, ANOVA with Bonferroni post-test). Error bars = SEM.