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. 2012 Nov 29;22(5):1017–1025. doi: 10.1093/hmg/dds506

Figure 4.

Figure 4.

COXI staining was used to determine mitochondrial function. Ten micrometer cryostat sections of embryos at E8.5 post coitis still enveloped in the maternally derived, deciduum at ×10 magnification and the inset panel at ×2.5 magnification. (A) A Polg2+/+ embryo and the maternal deciduum both stained positive for COXI activity. (B) A Polg2−/− embryo lacking COXI activity. (C) A Polg2+/+ embryo counterstained with hematoxylin showing a dual staining with COXI-positive cells. (D) A Polg2−/− embryo counter stained with hematoxylin showing COXI-negative cells stained in purple revealing the presence of an embryo in the deciduum.