Figure 5.

Clinical specimen analysis using DISSECT. Conventional PCR-Sanger sequencing (A) and conventional PCR-HRM (B) showing antisense TP53 exon 8 mutations from lung (c.830C>A, TL6 and c.818C>T, TL121) and colorectal (c.818C>T, CT20) clinical tumor samples, after three rounds of DISSECT. Results from DNA remaining on beads versus DNA eluted from beads are depicted (C) Plasma-circulating DNA from an esophageal cancer patient with a low-level mutation (c.847G>A, R283C) was analyzed by DISSECT followed by conventional or COLD-PCR-Sanger sequencing.