Table 1.
Sample | % D2O | Conc (mg/ml) | I(0) | MW (kDa) | Rg (Å) Guinier | Rg (Å) Gnom | Dmax (Å) | |
---|---|---|---|---|---|---|---|---|
SAXS | H-Mos1 | 0 | 1.8 | 13.5 | 79.9 | 49.2 ± 0.1 | 49.5 ± 0.2 | 185 |
SANS | H-Mos1 | 0 | 7.5 | 0.433 | 96.2 | 51.9 ± 0.7 | 53.4 ± 0.5 | 180 |
H-Mos1 | 100 | 8.8 | 0.913 | 89.5 | 51.2 ± 0.4 | 55.9 ± 0.2 | 180 | |
SANS | D-Mos1 | 0 | 1.1 | 0.38 | 86.4a | 51.5 ± 0.7 | 55.1 ± 0.6 | 180 |
D-Mos1 | 30 | 1.1 | 0.15 | 51.1 ± 1.5 | 53.7 ± 0.9 | 180 | ||
D-Mos1 | 65 | 1.2 | 0.049 | 53.7 ± 2.1 | 53.0 ± 2.1 | 180 | ||
D-Mos1 | 100 | 1.2 | 0.01 | |||||
Crystal Structure | Mos1 | 81.3 | 38b | 110 |
The coordinates for the Mos1 dimer in the PEC were extracted from the PDB file 3HOT.
aRenormalized for mass of H-Mos1.
bCaculated using CRYSOL.