Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Dec 18;41(3):1661–1668. doi: 10.1093/nar/gks1267

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

PMC Copyright notice

Figure 2. — The SprT domain of Spartan interacts with Pol δ subunit POLD3. (A) The E112A mutant of SprT captures Pol δ in vivo. Wild-type or the E112A mutant of SprT proteins were stably expressed in 293A and purified on anti-Flag beads. Copurified endogenous proteins were analysed by Western blotting for the indicated Pol δ subunits. (B) The SprT domain captures co-expressed POLD3 in vivo. Wild-type or the E112A mutant of SprT proteins were transiently expressed in 293T with each Pol δ subunit tagged with 4HA and immunoprecipitated with anti-Flag beads. Precipitated proteins were analysed by Western blotting for the indicated proteins. (C) Interactions between full-length Spartan and POLD3. Experiments were performed as in (B) except that full-length Spartan was expressed. (D) Direct interaction of the Spartan SprT domain with POLD3. In vitro GST-pull down assays were performed using the indicated recombinant proteins.