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. 2012 Dec 18;41(3):1661–1668. doi: 10.1093/nar/gks1267

Figure 4.

Figure 4.

Spartan depletion induces complex formation of POLD3 with Rev1 and Pol ζ. (A) Spartan depletion induces interaction of POLD3 with Rev1. Cells stably expressing 3xFlag-POLD3 at near physiological levels were transfected with the indicated siRNAs. 3xFlag-POLD3 proteins were immunoprecipitated and Western blotting was performed for the indicated proteins. RNAi of Rev1 shows the specificity of anti-Rev1 antibodies. #Cross-reactive bands. (B) Spartan depletion induces interaction of Rev1 and POLD3. Experiments were performed similarly in cells stably expressing 3xFlag-Rev1 after transfection with the indicated siRNAs. (C) Spartan depletion induces interaction of POLD3 with Rev7. Experiments were performed as in (A) and Western blotting was performed for the indicated proteins.