. 2012 Dec 24;41(3):2047–2059. doi: 10.1093/nar/gks1291

Table 2.

K_d values of DBD-F^M^B^P and various RPAs for RPA and p53TAD2 substrates as determined by either fluorescence quenching or fluorescence anisotropy

Protein	Substrate	K_d (µM)	SD (µM)	Method^a
RPA	FPA	29.0	3.5	1
RPA	p53TAD2	6.06	0.08	2
DBD-F	p53TAD2	1.03	0.25	1
DBD-F	FPA	9.0	1.2	2
RPAΔF	FPA	>400	NA	1
RPA(R41E,R43E)	FPA	230	18	1
DBD-F^b	p53TAD2	99.9	8.4	2
DBD-F^b	FPA	18.3	0.9	2

^a(1)Fluorescence quenching; (2)Fluorescence anisotropy.

^bFrom Souza-Fagundes et al. (63).