Figure 1.

Schematics of the three configurations used in the microtiter plate assays of this communication. (A) Binding assay with direct coating of purified RBPs (e.g. recombinant protein domains). (B) Binding assay with streptavidin immobilized pre-miRNAs and detection of RBP by antibodies. Source of RBP can be total cell lysate. (C) Inhibition assay for determining affinities of interactions. The assay is similar to that depicted in (B); the biotinylated RNA can be a short oligoribonucleotide known to bind the RBP.