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. 2012 Dec 15;41(3):1783–1796. doi: 10.1093/nar/gks1252

Figure 1.

Figure 1.

Genomic SELEX experiment with the multidomain cyclophilin AtCyp59_RRM_Zn domain. (a) Scheme of Arabidopsis RNA library preparation and selection process. (b) Schematic representation of the domain organization of the full-length AtCyp59 and GST-tagged construct of the RRM+Zn motif of AtCyp59. PPIase, peptidyl/prolyl cis/trans isomerase domain; RRM, RNA recognition motif; Zn, zinc finger motif class CCHC; RS/RD(E), domain enriched in arginine, serine, aspartate, glutamate; GST, glutathione S-transferase. (с) Enrichment of the RNA sequences from the Arabidopsis thaliana genome which bind AtCyp59. The RNA recovery was calculated as a percentage of the RNA material that was bound to the protein relatively to the amount of the initial RNA material in each cycle. During Cycles 1–3, the molecular ratio of the RNA to protein was 3:1, Cycles 4–9, 11 the ratio was 10:1, and Cycle 10 anti-GST selection.