Table 1.
Range of dissociation constants of 17 different 7-nt RNA variants of the binding motif found in genomic SELEX and AtCyp59_RRM_Zn domain based on gel shift assay
| Sequence | Kd AtCyp59_RRM_Zn (nM) | Kd AtCyp59 (nM) |
|---|---|---|
| GGUGCCG | 40 ± 10 | 120 ± 25 |
| GUGGCCG | 40 ± 10 | 120 ± 25 |
| GUCGCCG | 40 ± 10 | 120 ± 25 |
| GUAGCCA | 105 ± 25 | 120 ± 25 |
| GCUGCCG | 105 ± 25 | 120 ± 25 |
| GAUGCCA | 105 ± 25 | 120 ± 25 |
| GACGCCA | 105 ± 25 | 120 ± 25 |
| GCCGCCG | 200 ± 35 | 120 ± 25 |
| GUUGCCG | 200 ± 35 | 120 ± 25 |
| GUAGCCG | 200 ± 35 | 120 ± 25 |
| GCGGCCG | 200 ± 35 | 120 ± 25 |
| GUCGCCA | 300 ± 40 | 120 ± 25 |
| GCCGCCA | 300 ± 40 | 120 ± 25 |
| GAUGCCG | 300 ± 40 | 120 ± 25 |
| GUGGCCA | 300 ± 40 | 120 ± 25 |
| GGAGCCA | 300 ± 40 | 120 ± 25 |
| GCGGCCA | 300 ± 40 | 120 ± 25 |