Figure 5.

Overexpression of AHR but not ARNT prevented TiPARP-mediated inhibition of AHR transactivation. HuH-7 cells were co-transfected with pCYP1A1-luc, TiPARP (200 ng) and (A) AHR (250–750 ng) or (B) ARNT. Results represented the means ± S.E.M from three independent experiments and were analysed by one-way ANOVA and Tukey’s multiple comparisons test. Reporter gene activity of cells co-overexpressing TiPARP significantly lower than (P < 0.05) transfection- and treatment-matched control cells was denoted with an asterisk. Western blots confirming the presence of increased AHR and ARNT protein expression following transient transfection were presented to the right of the respective bar graphs.