Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| N16961 | O1 El Tor, Inaba | Laboratory stock |
| N16961-Sm | Spontaneous mutant of N16961; Smr | This study |
| 919C | O1 El Tor | Laboratory stock |
| V07-16 | O1 El Tor, Inaba | Laboratory stock |
| 367 | O1 El Tor, Ogawa | Laboratory stock |
| V06-92 | O1 El Tor, Ogawa | Laboratory stock |
| 465 | O1 El Tor, Inaba | Laboratory stock |
| 470 | O1 El Tor, Ogawa | Laboratory stock |
| 417 | O1 El Tor, Inaba | Laboratory stock |
| 136 | O1 El Tor, Ogawa | Laboratory stock |
| 427-2A1 | N16961-Sm,vc0241::Tn, Smr Kanr VP4r | This study |
| 514-2G8 | N16961-Sm,vc0241::Tn, Smr Kanr VP4r | This study |
| 514-3F2 | N16961-Sm,vc0251::Tn, Smr Kanr VP4r | This study |
| 513-2E4 | N16961-Sm,vc0251::Tn, Smr Kanr VP4r | This study |
| 516-8B8 | N16961-Sm,vc0242::Tn, Smr Kanr VP4r | This study |
| 506-1C4 | N16961-Sm,vc0245::Tn, Smr Kanr VP4r | This study |
| 514-4C2 | N16961-Sm,vc0243::Tn, Smr Kanr VP4r | This study |
| 427-2H5 | N16961-Sm,vc0260::Tn, Smr Kanr VP4r | This study |
| 506-2B11 | N16961-Sm,vc2417::Tn, Smr Kanr VP4r | This study |
| N16961-Sm ΔmanB | manB deletion of N16961-Sm | This study |
| N16961-Sm ΔwbeU | wbeU deletion of N16961-Sm | This study |
| N16961-Sm ΔmanB/CmanB | manB complementation of N16961-Sm ΔmanB | This study |
| N16961-Sm ΔwbeU/CwbeU | wbeU complementation of N16961-Sm ΔwbeU | This study |
| 95001 CwbeE/CwbeU | wbeE and wbeU complementation of 95001 | This study |
| 367 CmanB | manB complementation of 367 | This study |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km λpir | 47 |
| Plasmids | ||
| pSC123 | Suicide plasmid carrying transposon; Kanr Cmr | 9 |
| pWM91 | Suicide plasmid; oriR oriT lacZ tetAR sacB | 40 |
| pWM91Δ0242 | pWM91 carrying upstream and downstream fragments flanking manB | This study |
| pWM91Δ2417 | pWM91 carrying upstream and downstream fragments flanking recJ | This study |
| pCVD442 | Suicide plasmid; R6K ori mob RP4 bla sacB | 13 |
| pCVD442-Δvc0260 | pCVD442 carrying upstream and downstream fragments flanking wbeU | This study |
| pBR322 | Cloning vector; ColE1 ori Ampr Tetr | TaKaRa |
| pBR322-c0260 | pBr322 carrying wbeU | |
| pACYC184 | Cloning vector; rep Cmr Tcr | 6 |
| pACYC184-c0242 | pACYC184 carrying manB | This study |
| pACYC184-c0244 | pACYC184 carrying wbeE | This study |
| pACYC184-c0261 | pACYC184 carrying wbeW | This study |
| pUC18 | Cloning vector; ori lacZ Ampr | TaKaRa |
| pUC18-c0260 | pUC18 carrying wbeU | This study |