Table 3.

Mutations and gene complementation of natural isolates resistant or sensitive to phage VP4

Strain	Sensitivitya	Amino acid substitution in wb* cluster genes	Gene(s) used to complement	Sensitivity after complementation
367	R	manB, I72V, I77L, Y91H; wbeW, A13V, A118V, S122A	manB	Restored
V07-16	R	manB, I72V, I77L, Y91H; wbeW, A13V, A118V, S122A	manB; manB+wbeW	Not restored
V06-92	R	manB, I72V, I77L, Y91H; wbeW, A13V, A118V, S122A	manB; manB+wbeW	Not restored
95001	R	wbeU, 12-base fragment deletion of ntb 1670–1681; wbeE, single-nucleotide deletion of nt 393, codon TAA occurs in nt 413; manC, S54P	wbeE+wbeU	Restored
465	S	wbeW, A13V, A118V, S122A
470	S	wbeW, A13V, A118V, S122A
417	S	wbeW, A13V, A118V, S122A
136	S	wbeW, A13V, A118V, S122A

^aR, resistant to VP4; S, sensitive to VP4.

^bnt, nucleotide.