Fig 4.

Absolute temperature does not control production of OspC or Erp proteins. B. burgdorferi bacteria were cultured in complete BSK-II medium under various temperature regimens, and protein contents were examined by immunoblotting. Conditions examined were cultures inoculated with bacteria that had been frozen for >1 month at −80°C and then cultured at 23°C (left lanes), cultures inoculated with bacteria that had been grown to late exponential phase at 34°C and then cultured at 23°C (center lanes), and cultures inoculated with bacteria that had been grown to late exponential phase at 23°C and then cultured at 34°C (right lanes). All cultures were harvested at late exponential phase. The constitutively expressed flagellar component FlaB served as a control, and fold changes of other proteins were calculated relative to the FlaB band intensities (44). Illustrated data for each condition are from analyses of the same paired bacterial lysates.