Fig 2.

RMI is extracellular and requires coculturing of PS01 and PS02. Cell-free supernatants were obtained from PS01, PS02, and a coculture of both strains (labeled “Supe”) grown in 2216 broth. RMI activity was measured using a fliC3p::lacZ transcriptional fusion reporter strain (PS02/pPS1) and is reported as the β-galactosidase activity in Miller units. Controls: medium-only blank (Control) and monoculture supernatants from PS01 and PS02 separately and mixed 1:1 (PS01+PS02). The means plus the standard deviations are shown from three independent experiments. The asterisk indicates the cocultured supernatant is statistically significant from the controls, as determined by using the Student t test (P < 0.001).