Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F− endA1 hsdR17(rK− mK−)supE44 thi-1 recA1 gyrA96 relA1 ϕ80dlacZΔM15 | 27 |
| S17-1λpir | thi pro recA hsd(r− m+) RP4-2 Tc::Mu-Km::Tn7 Smr λpir | 28 |
| Roseobacter | ||
| Silicibacter sp. strain TM1040 | Wild type | 29 |
| HG1016 | flaC::EZ-Tn5; Kanr | 15 |
| HG1006 | flaB::EZ-Tn5; Kanr | 15 |
| HG1038 | ctrA::EZ-Tn5; Kanr | 17 |
| HG1310 | tdaA::EZ-Tn5; Kanr | 17 |
| HG1015 | tdaB::EZ-Tn5; Kanr | 17 |
| HG1265 | tdaE::EZ-Tn5, Kanr | 17 |
| HG1050 | tdaF::EZ-Tn5, Kanr | 17 |
| HG1244 | tdaH::EZ-Tn5; Kanr | 17, 18 |
| PS01 | flaC pigmented | This study |
| PS02 | flaC nonpigmented | This study |
| Plasmids | ||
| pEA203 | cdhA′::lacZ Ori pC2A Ampr Purr Ori R6K | 30 |
| pRK415 | Conjugative expression shuttle vector for Silicibacter sp. strain TM1040 and E. coli; Plac; Tetr | 31 |
| pPS1 | 300 bp upstream of fliC3 fused to promoterless lacZ gene in pRK415 | This study |
| pRSI506 | pRK415 vector with 1,022 bp of genomic DNA containing 235 bp upstream and 100 bp downstream of flaC | 15 |
a
Kanr, kanamycin resistance; Smr, streptomycin resistance; Tetr, tetracycline resistance; Ampr, ampicillin resistance; Purr, puromycin resistance.