Table 5.

RMI activity in paa and tda mutant backgrounds

Strain	Function	Mean β-Galactosidase activitya	% β-Galactosidaseb
paaI mutant	Phenyl oxygenase	26 ± 2.10	39
paaJ mutant	Phenyl oxygenase	42 ± 1.05	63
paaK mutant	Phenyl oxidoreductase	39 ± 0.33	59
tdaA mutant	LysR substrate binding domain protein	72 ± 0.70	109
tdaB mutant	β-Etherase, GST	80 ± 2.30	121
tdaE mutant	Acyl-coenzyme A dehydrogenase (ACAD)	58 ± 1.60	88
tdaF mutant	Phosphopantothenoylcysteine decarboxylase	60 ± 1.40	91
tdaH mutant	Sulfite oxidase domain protein	67 ± 1.20	100
Control		66 ± 2.20	100

^aRMI activity was measured using a fliC3p::lacZ reporter strain, PS02/pPS1, and is expressed as the β-galactosidase activity in Miller units. The means obtained from three independent experiments are presented.

^bThat is, the percent increases from control (cell-free RMI supernatant obtained from TM1040 culture in 2216 broth).