Table 3.
PlevD-cat activities measured in various strains under carbohydrate pulsinga
| Pulsing sugar(s) and strain | Avg CAT sp act (SD) when pulsed with indicated concn of sugar |
|||||
|---|---|---|---|---|---|---|
| 0 mM | 0.05 mM | 0.2 mM | 1 mM | 5 mM | 10 mM | |
| Sucrose | ||||||
| MMZ998 | 15.8 (5.9) | 22.4 (4.9) | 117.3 (12) | 308.7 (37) | 169.4 (18) | 185.1 (12) |
| MMZ1002 | 17.3 (6.2) | 14.2 (5.7) | 12.5b (4.4) | 16.4b (5.0) | 24.0b (7.1) | 31.0b (7.3) |
| MMZ998Q | 16.3 (1.3) | ND | 9.3b (0.5) | 9.6b (1.1) | 2.2b (0.3) | ND |
| MMZ998T | 242b (6.4) | ND | 247b (8.5) | 242 (43) | 37.1b (1.1) | ND |
| MMZ998R | 0b (0) | ND | 0.23b (0.2) | 0.25b (0.2) | 0.18b (0.04) | ND |
| Fructose | ||||||
| MMZ998 | ND | 190 (6.8) | 387 (7.5) | 511 (12) | ND | |
| MMZ1002 | ND | 180 (15) | 388 (38) | 486 (93) | ND | |
| Mannose | ||||||
| MMZ998 | ND | 251 (6.5) | 564 (30) | 359 (6.3) | ND | |
| MMZ1002 | ND | 241 (29) | 557 (97) | 471 (81) | ND | |
| Glucose and fructose | ||||||
| MMZ998 | ND | 258 (11) | 556 (2.1) | 677 (33) | ND | |
| MMZ1002 | ND | 281 (46) | 651 (114) | 692 (179) | ND | |
a
The cells were cultured in TV containing 0.5% galactose to an OD600 of 0.2, and then the indicated concentrations of carbohydrates were added. Cells were then incubated for 3.5 h before CAT assays. CAT activity is expressed as nmol of chloramphenicol acetylated (mg of protein)−1 (min)−1. The data are averages from three independent cultures. ND, not determined.
b
The data are statistically different from those obtained for MMZ998 under corresponding conditions as verified by Student's t test (P < 0.05).