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. 2013 Feb;195(4):765–776. doi: 10.1128/JB.01639-12

Table 1.

Growth of Fpv− mutants of Pf-5 on an iron-limited medium in the presence of cross-feeding strains of Pseudomonas spp.

Cross-feeding strain Growth of Pf-5 deletion mutanta:
 Peptide chain compositionb Reference(s) or source
fpvZ fpvU fpvY fpvW fpvX fpvV
P. protegens Pf-5e − + + + + + Asp-FOHOrn-Lys-(Thr-Ala-Ala-FOHOrn-Lys) This study
P. protegens CHA0e − + + + + + Asp-FOHOrn-Lys-(Thr-Ala-Ala-FOHOrn-Lys) 26
P. chlororaphis DTR133 − + + + + + Asp-FOHOrn-Lys-(Thr-Ala-Ala-FOHOrn-Ala) 29
P. aeruginosa PAO1e + − + + + + Ser-Arg-Ser-FOHOrn-(Lys-FOHOrn-Thr-Thr) (type I pyoverdine) 30
P. fluorescens SBW25e + − + + + + Ser-Lys-Gly-FOHOrn-(Lys-FOHOrn-Ser) 31
P. fluorescens ATCC 13525 + − + + + + Ser-Lys-Gly-FOHOrn-(Lys-FOHOrn-Ser) 32
P. chlororaphis ATCC 9446 + − + + + + Ser-Lys-Gly-FOHOrn-(Lys-FOHOrn-Ser) 33
P. rhodesiae CFML 92-104 + + − + + + Ser-Lys-FOHOrn-Ser-Ser-Gly-(Lys-FOHOrn-Ser–Ser)c 6, 34
P. rhodesiae DSM14020 + + − + + + Ser-Lys-FOHOrn-Ser-Ser-Gly-(Lys-FOHOrn-Ser-Ser)c 6, 34
P. marginalis pv. alfalfa NCPPB 2644 + + − + + + Unknown 35
P. marginalis pv. marginalis NCPPB 667 + + − + + + Unknown 35
P. marginalis pv. pastinacae NCPPB 806 + + − + + + Unknown 35
Pseudomonas sp. B10 + + + − + + εLys-OHAsp-Ala-aThr-Ala-cOHOrn 36
P. lini DLE411J + + + − + + Lys-OHAsp-Ala-Thr-Ala-OHOrnc 34, 37
P. fluorescens ATCC 17513 + + + − + + Unknown 38
P. putida CS111 + + + + − + Ala-Lys-Thr-Ser-OHOrn-OHOrnc 39
Pseudomonas sp. SB8.3 + + + + − + Ala-Lys-Thr-Ser-AcOHOrn-cOHOrn 5
P. putida Bn7 + + + + + − Unknown 40
P. fluorescens A506e + + + + + + Ser-Lys-Gly-FOHOrn-(Lys-FOHOrn-Ser)d This study
P. fluorescens WCS374e + + + + + + Ser-Lys-Gly-FOHOrn-(Lys-FOHOrn-Ser)c 41
P. fluorescens CLR711f + + + + + + Ser-AcOHOrn-Ala-Gly-aThr-Ala-cOHOrn 42
P. costantinii CFBP 5705 + + + + + + Ser-AcOHOrn-Gly- Thr-aThr-Gln-Gly-Ser-cOHOrn 43
P. fluorescens CTRp112f + + + + + + Lys-AcOHOrn-Ala-Gly-aThr-Ser-cOHOrn 42
Pseudomonas sp. A6 + + + + + + Lys-AcOHOrn-Gly-aThr-Thr-Gln-Gly-Ser-cOHOrn 44
P. putida CFML90-40 + + + + + + Asp-Ala-Asp-AcOHOrn-Ser-cOHOrn 39, 45
P. aeruginosa ATCC 27853 + + + + + + Ser-FOHOrn-Orn-Gly-aThr-Ser-cOHOrn (type II pyoverdine) 46
P. aeruginosa 7NSK2 + + + + + + Ser-FOHOrn-Orn-Gly-aThr-Ser-cOHOrn (type II pyoverdine) 47
a

Growth of Fpv− mutants in a ΔpvdI-pchC background of Pf-5 on an iron-limited medium (KMB amended with 400 or 600 μM 2,2′-dipyridyl) in the presence of the cross-feeding strain: +, growth; −, no growth. The ΔpvdI-pchC mutant of Pf-5 did not grow on the iron-limited medium in isolation but did grow in the presence of all cross-feeding strains listed. Values represent results from two replicate plates of at least two independent experiments.

b

Composition of the peptide chain of the pyoverdine produced by the cross-feeding strain. An underline denotes a d-amino acid. Parentheses define cyclic residues. cOHOrn, cyclo-hydroxy-ornithine; εLys, Lys linked by its ε-NH2; OHAsp, threo-β-hydroxy-aspartic acid; aThr, allo-Thr; AcOHOrn, δN-acetyl-δN-hydroxy-ornithine.

c

Incomplete structures lacking stereochemistry. Italicized peptide chains are inferred from siderotyping analysis (6). These pyoverdines are in the same siderotype as a pyoverdine with a chemically determined structure. Predicted pyoverdine structures do not show amino acid modifications or stereochemistry, as these are unknown.

d

Structures predicted from bioinformatic analysis of the NRPS sequences in the pyoverdine gene clusters in the genomes of these strains.

e

Strains known to produce secondary siderophores, in addition to pyoverdines: P. protegens Pf-5 and CHA0, enantio-pyochelin (4); P. aeruginosa PAO1 and 7NSK2, pyochelin (7, 48); P. fluorescens SBW25, ornicorrugatin (7); P. fluorescens WCS374 and A506, pseudomonine (41, 49). Pf-5 can utilize enantio-pyochelin as an iron source, but it cannot utilize pyochelin (50) and lacks the TBDPs for ornicorrugatin and pseudomonine (E. W. Davis II, S. L. Hartney, and J. E. Loper, unpublished data).

f

Strain CTRp112 is the same as strain PL8 and strain CLR711 is the same as strain PL7 (J. M. Meyer, personal communication), and the structures of pyoverdines produced by strains PL8 and PL7 are known (42).