Fig 3.

Enrichment of normally shaped cells from E. coli CS315-1K after 15 rounds of FACS sorting. (A to C) Histograms of forward light scatter CS109 (A), CS315-1K (B), and CS315-1K-15F (C). This was the population distribution after 15 rounds of enrichment sorting, and it was from this population that E. coli ALM10 was isolated. (D to F) Phase-contrast microscopy of cells treated with aztreonam (5 μg/ml) filaments the cells and accentuates any abnormalities: CS109 (D), CS315-1K (E), and CS315-1K-15F (F). The lack of branching in panel F indicates that the sorted population was enriched for normal rod-shaped cells.